Fig. 4.

Supplementation of LG medium with βHB does not affect proliferation of HeLa cells with downregulated BDH1 and OXCT1. A: Proliferation of siR-NC HeLa cells cultured for 72 h in βHB-supplemented LG medium was significantly higher than that of cells cultured in LG medium alone (*P < 0.05 and ***P < 0.001 for 5 mM and 10 mM βHB, respectively. B, C: Proliferation of siR-BDH1 and siR-OXCT1 HeLa cells was increased in the presence of 5 mM or 10 mM βHB in LG medium (*P < 0.05). D: Proliferation of siRNA-BDH1-and-OXCT1 HeLa cells was not affected by βHB supplementation in LG medium with βHB. Values are indicated as mean ± SD of three separate experiments.