Fig. 2.

The 2-BrHDA reacts with GSH to form HDA-GSH. Reaction products of GSH (1 µmol) with 1 µmol of either HDA, 2-ClHDA, 2-ClHOH, 2-ClHA, 2-BrHDA, or 2-BrHA were visualized by phosphomolybdic acid staining following TLC chromatography as described in the Materials and Methods (A). TLC purified reaction products from 2-BrHDA and GSH were subsequently analyzed by ESI-MS/MS. Both the Q1+ parent molecular ion at m/z of 546.3 [(B) insert] and CAD analyses of m/z 546.3 (B) were the same as that previously reported for the reaction product of 2-ClHDA (12) and assignments for the depicted molecular ions in (B) are provided in (C).