Fig. 6.

The 2-BrFALD and FALD-GSH in mice exposed to bromine gas. C57bl/6 mice were exposed to Br₂ gas (400 or 600 ppm, 30 min) and then brought back to room air. At 1 h following exposure, lungs and plasma were collected. The 2-BrFALD (A) and FALD-GSH (B) were then measured in the lung and FALD-GSH (C) was measured in the plasma. FALD-GSH was extracted from the lung tissue and plasma and quantitated using ESI-MS/MS selective reaction monitoring as described in the Materials and Methods. *P < 0.05, **P < 0.005, and ***P < 0.001 for production of FALD-GSH compared with air controls; n = 3 for each treatment.