Fig. 7.

NEM blocks 2-BrHDyA and 2-ClHDyA reaction with THP-1 cell protein. THP-1 cells were pretreated with or without 1 mM NEM or 5 mM NAC for 30 min at 37°C followed by treatment with 5 μM HDyA, 2-ClHDyA, or 2-BrHDyA for 30 min at 37°C. TAMRA-clicked THP-1 cell protein was visualized after gel electrophoresis. Panel A shows Coomassie blue total protein stain. NEM pretreatment blocked the fluorescence signal of 2-BrHDyA and 2-ClHDyA, but NAC pretreatment only significantly decreased the 2-BrHDyA signal (B).