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Connecting synthetic receptors to downstream genetic circuits. (A) General architecture of LexA-VHH-Caffeine connected to the BetI inverter. LexA-VHH-Caffeine controls BetI expression, which controls the GFP expression. (B) Response of cells containing the LexA-VHH-Caffeine receptor connected to the BetI inverter to increasing concentrations of IPTG and caffeine. (C) Fold activation of the LexA-VHH-Caffeine/BetI inverter circuit.
