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. 2018 Jan 22;140(7):2493–2503. doi: 10.1021/jacs.7b10439

Figure 3.

Figure 3

Different types of Ure2 oligomers revealed by confocal single molecule FRET. (A,B) SmFRET efficiency distribution of selected oligomers of AF488/AF647-labeled Ure2 at different incubation times. The FRET distributions at different time points were fitted globally to double Gaussian functions giving the average peak positions indicated. (C,D) Population of low- and high-FRET oligomers of Ure2 at different incubation times. The incubation conditions were the same as in Figure 1. (A,C) Ure2-S53C. (B,D) Ure2-V9C.