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Detection performance parameters of the CST4-ELISA system
| Detection performance | Parameters |
|---|---|
| Linear range | 50–1,600 pg·mL−1 |
| Hook effect | 6,400 pg·mL−1a |
| LOD | 40 pg·mL−1 |
| Precision (batch-to-batch CV) | 3.69% |
| Precision (day-to-day CV) | 4.43% |
| Recovery rate | 80%–120% |
Note:
Complete saturation of the signal was not seen till 6,400 pg·mL−1.
Abbreviations: CST4, cystatin S; CV, coefficient of variation; ELISA, enzyme-linked immunosorbent assay; LOD, limit of detection.
