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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Eur J Immunol. 2017 Aug 21;47(11):1890–1899. doi: 10.1002/eji.201747113

Figure 1.

Figure 1

CpG-B-DOTAP is an effective adjuvant for T cell-dependent antigens. A-B) Mice were immunized intraperitoneally with NP-CGG (100 μg/mouse) either alone or with CpG-A or CpG-B with or without DOTAP (70 μg/mouse CpG +/− 190 μg/mouse DOTAP) in HBS. Control mice received 500 μl HBS only. Spleens were taken 14 days post injection and analyzed by flow cytometry according to the gating strategy described in Supplementary Figure 1. Representative flow cytometry plots indicate the relative frequencies of: A) GC B cells among total B cells or B) Tfh cells among total CD4+ T cells. Total number of: C) GC B cells; D) NP-specific GC B cells and E) Tfh cells are shown. Values that are significantly different from those of mice immunized with NP-CGG alone are shown with asterisks (0.01 <P ≤0.05 = *; 0.001 <P ≤0.01 = **) (t-test). Data shown are representatives of three independent experiments each with 5 mice per condition.