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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Eur J Immunol. 2017 Aug 21;47(11):1890–1899. doi: 10.1002/eji.201747113

Figure 3.

Figure 3

CpG-B-DOTAP enhances plasma cell generation in response to NP-CGG immunization. WT mice were immunized as described in Figure 1 and spleens were harvested 14 days post injection. A) Flow cytometry plots indicating the frequency of PCs and PBs among live splenocytes are shown. B) Representative images demonstrating the PCs secreting IgG NP-specific Ab, as detected by plating equal numbers of splenocytes on ELISPOT plates coated with either NP(30)BSA (top panel) or NP(4)BSA (bottom panel), are shown. C) Shown are the total numbers of PCs and PBs as detected by flow cytometry, NP-specific PCs as detected by either (D) NP(30)BSA ELISPOT or (E) NP(4)ELISPOT are shown. Values that are significantly different compared to those of mice immunized with NP-CGG alone are shown with asterisks (0.01 <P ≤0.05 = *; 0.001 <P ≤0.01 = **) (t-test). Data represents three independent experiments each containing five mice per group