Figure 1.

Store depletion induces Ca²⁺ entry in hTrp3-transfected wild-type DT40 cells. Fura-2-loaded wild-type DT40 cells transfected with either hTrp3 or its vector (Mock), along with a construct encoding eGFP as transfection marker (see Materials and Methods), were incubated in a nominally Ca²⁺-free medium and then exposed to 2 μM thapsigargin to deplete intracellular Ca²⁺ stores. After cytosolic Ca²⁺ returned to basal levels, Ca²⁺ (1.5 mM) was re-added to the medium. Shown are average curves ± SEM (in gray) from three independent experiments, each of them performed on at least four single cells. Measurements were performed on positively transfected cells, which were selected by their green fluorescence when excited at 488 nm (eGFP-positive cells).