Figure 3.

Store depletion induces Ba²⁺ entry in hTrp3-transfected wild-type and IP₃R-KO DT40 cells. Ba²⁺ influx was measured in Fura-2-loaded DT40 cells transfected with either hTrp3 (wild type, IP₃R-KO) or its vector (pcDNA3, Mock, wild type only) as described in Fig. 1 legend. The cells were maintained in a nominally Ca²⁺-free medium, exposed to 2 μM thapsigargin, and then Ba²⁺ (10 mM) was added where indicated. Shown are average curves ± SEM (in gray) from four independent experiments, each of them performed on at least four single cells.