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. 2018 Feb 24;9(20):15326–15339. doi: 10.18632/oncotarget.24569

Figure 1.

Figure 1

(A) Growth characteristics of tumor xenografts derived from genetically engineered 231-EV (n = 9, diamond), sh-HIF-1α (n = 10, triangle), sh-HIF-2α (n = 9, square) and sh-HIF-1/2α (n = 7, dot) cells. Growth curves were generated by averaging the tumor volume of mice in each group. (B) RT q-PCR analysis showing HIF-1α and HIF-2α mRNA expression levels in 231-EV (n = 8), sh-HIF-1α (n = 10), sh-HIF-2α (7-8) and sh-HIF-1/2α (n = 5) tumors. Values represents Mean fold induction ± SEM of the ∆Ct Values. P-values less than 0.05 were considered significant. *p ≤ 0.05, *** p ≤ 0.0005. (C) Representative immunoblots showing HIF-1α and HIF-2α levels in 231-EV, sh-HIF-1α, sh-HIF-2α and sh-HIF-1/2α tumors. GAPDH was used as a loading control in the western blots.