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Representative (A) total choline and (B) lipid maps of tumors from 231-EV, sh-HIF-1α, sh-HIF-2α and sh-HIF-1/2α tumors. (C) Corresponding in vivo MR spectra showing choline and lipid signals at 3.2 and 1.0 ppm respectively. Bar plot showing quantitative analysis of (D) total choline and (E) lipids. Values represent mean ± SEM. *p ≤ 0.05. The total choline signal consists of free choline, phosphocholine and glycerophosphocholine. The lipid signal may contain signal from lactate.
