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. Author manuscript; available in PMC: 2018 Apr 2.
Published in final edited form as: Int J Oncol. 2010 Sep;37(3):737–743. doi: 10.3892/ijo_00000723

Figure 3.

Figure 3

Influence of pulse-exposure to CuB on growth and cell morphology of CSCC cells. (A) SRB12 cells were exposed to CuB (10-7 M) for 2, 9 or 24 h, washed extensively, cultured in CuB-free culture medium for 24, 48 and 72 h, and cell growth was measured by MTT assay. Each point represents a mean ± SD of triplicate wells. (B) CuB causes multinucleation: SRB12 cells were treated with CuB (2.5×10-7 M for 2 days), washed and cultured an additional 2 days with no CuB, multinucleation developed, see arrows. (C) SRB1 or SRB12 cells were cultured with CuB (10-6 M or 10-7 M for 24 h), washed extensively and cultured in regular media for an additional 24 h and examined for DNA content per cell by FACS after staining with propidium iodine. Cells were gated using FL2-W versus FL2-A bivariant graphs.