Table I.
Primer sequences and concentrations used for quantitative real-time polymerase chain reaction.
| Primer | Sequence | Concentration |
|---|---|---|
| Cyclophilin-A | 5′-GTGTTCTTCGACATCACGGCT-3′ (forward) 5′-CTGTCTTTGGAACTTTGTCTGCA-3′ (reverse) |
200 nM |
| HCRTr1 | 5′-GCGAGGTCCAGGGCTCACT-3′ (forward) 5′-GCCTGGTACACCCAGATGCCG-3′ (reverse) |
100 nM |
| HCRTr2 | 5′-GGCAGGACCGCTTCAAAACTGGTAT-3′ (forward) 5′-CCCTCAGACCTCCAGCCAGGT-3′ (reverse) |
200 nM |
| TH | 5′-CCTTCCAGTACAAGCACGGT-3′ (forward) 5′-TGGGTAGCATAGAGGCCCTT-3′ (reverse) |
100 nM |
| VMAT | 5′-AGTCTCTGCTGTCTTGCCAAC-3′ (forward) 5′-TGGTCCAGTGAAGCTCACTTCT-3′ (reverse) |
200 nM |
| D2R | 5′-AGACGATGAGCCGCAGAAAG-3′ (forward) 5′-GCAGCCAGCAGATGATGAAC-3′ (reverse) |
100 nM |
| DAT | 5′-CAACGGTGGCATCTACGTCT-3′ (forward) 5′-CTGAATTGCTGGACGCCGTA-3′ (reverse) |
50 nM |