Table 2.
Associations between derived IgG Fc-glycosylation traits and IBD in the ITA cohort.
| HC vs. UCb | HC vs. CD | UC vs. CD | |||||
|---|---|---|---|---|---|---|---|
| Derived traita | Description | OR (95% CI)c | p-valued | OR (95% CI) | p-value | OR (95% CI) | p-value |
| IgG1 | |||||||
|
| |||||||
| IgG1_agal | Fraction of agalactosylated glycans | 1.29 (1.13 – 1.47) | 8.9E-05 | 1.69 (1.46 – 1.96) | 2.2E-13 | 1.23 (1.11 – 1.36) | 3.9E-05 |
| IgG1_digal | Fraction of digalactosylated glycans | 0.88 (0.77 – 1.00) | 5.7E-02 | 0.61 (0.52 – 0.70) | 2.7E-12 | 0.71 (0.64 – 0.79) | 8.0E-11 |
| IgG1_monogal | Fraction of monogalactosylated glycans | 0.69 (0.61 – 0.79) | 5.4E-09 | 0.69 (0.61 – 0.79) | 4.3E-08 | 1.06 (0.97 – 1.17) | 2.0E-01 |
| IgG1_sial | Fraction of sialylated glycans | 1.05 (0.93 – 1.20) | 4.1E-01 | 0.81 (0.70 – 0.92) | 1.4E-03 | 0.78 (0.71 – 0.87) | 1.5E-06 |
| IgG1_A2B | Bisection of diantennary glycans | 0.69 (0.61 – 0.78) | 3.5E-09 | 0.91 (0.79 – 1.03) | 1.4E-01 | 1.22 (1.11 – 1.35) | 7.8E-05 |
| IgG1_A2F | Fucosylation of diantennary glycans | 0.93 (0.82 – 1.04) | 2.1E-01 | 1.27 (1.12 – 1.44) | 2.2E-04 | 1.36 (1.23 – 1.51) | 6.0E-10 |
| IgG1_A2G | Galactosylation per antenna on diantennary glycans | 0.81 (0.71 – 0.92) | 1.5E-03 | 0.59 (0.51 – 0.69) | 3.2E-13 | 0.77 (0.69 – 0.85) | 2.5E-07 |
| IgG1_A2S | Sialylation per antenna on diantennary glycans | 1.04 (0.92 – 1.18) | 5.1E-01 | 0.78 (0.68 – 0.90) | 3.2E-04 | 0.77 (0.70 – 0.85) | 3.7E-07 |
| IgG1_A2GS | Sialylation per galactose on diantennary glycans | 1.33 (1.18 – 1.51) | 3.6E-06 | 1.15 (1.01 – 1.30) | 3.1E-02 | 0.85 (0.77 – 0.94) | 1.1E-03 |
|
| |||||||
| IgG23 | |||||||
|
| |||||||
| IgG23_agal | Fraction of agalactosylated glycans | 1.30 (1.14 – 1.50) | 1.3E-04 | 2.76 (2.31 – 3.29) | 1.7E-37 | 2.11 (1.87 – 2.37) | 1.2E-40 |
| IgG23_digal | Fraction of digalactosylated glycans | 0.82 (0.71 – 0.94) | 3.7E-03 | 0.41 (0.35 – 0.48) | 2.2E-31 | 0.49 (0.43 – 0.55) | 1.0E-36 |
| IgG23_monogal | Fraction of monogalactosylated glycans | 0.77 (0.67 – 0.87) | 4.1E-05 | 0.39 (0.32 – 0.46) | 8.5E-34 | 0.54 (0.48 – 0.60) | 8.2E-32 |
| IgG23_sial | Fraction of sialylated glycans | 0.87 (0.76 – 0.99) | 2.9E-02 | 0.53 (0.46 – 0.61) | 6.8E-20 | 0.58 (0.52 – 0.64) | 1.0E-24 |
| IgG23_A2B | Bisection of diantennary glycans | 0.62 (0.55 – 0.71) | 3.2E-13 | 0.91 (0.80 – 1.05) | 1.9E-01 | 1.34 (1.21 – 1.48) | 8.2E-09 |
| IgG23_A2F | Fucosylation of diantennary glycans | 0.72 (0.63 – 0.82) | 3.2E-07 | 1.12 (0.99 – 1.27) | 7.5E-02 | 1.57 (1.41 – 1.74) | 1.6E-18 |
| IgG23_A2G | Galactosylation per antenna on diantennary glycans | 0.79 (0.68 – 0.90) | 5.3E-04 | 0.38 (0.32 – 0.45) | 1.9E-35 | 0.47 (0.42 – 0.53) | 4.1E-40 |
| IgG23_A2S | Sialylation per antenna on diantennary glycans | 0.86 (0.76 – 0.98) | 2.3E-02 | 0.52 (0.45 – 0.60) | 5.7E-21 | 0.57 (0.51 – 0.64) | 2.4E-25 |
| IgG23_A2GS | Sialylation per galactose on diantennary glycans | 1.02 (0.90 – 1.14) | 8.0E-01 | 1.19 (1.05 – 1.35) | 7.6E-03 | 1.11 (1.01 – 1.22) | 2.9E-02 |
|
| |||||||
| IgG4 | |||||||
|
| |||||||
| IgG4_agal | Fraction of agalactosylated glycans | 1.30 (1.13 – 1.48) | 1.2E-04 | 2.16 (1.84 – 2.54) | 2.3E-24 | 1.62 (1.46 – 1.80) | 3.7E-20 |
| IgG4_digal | Fraction of digalactosylated glycans | 0.89 (0.78 – 1.01) | 7.9E-02 | 0.53 (0.46 – 0.61) | 2.8E-18 | 0.60 (0.53 – 0.67) | 1.4E-21 |
| IgG4_monogal | Fraction of monogalactosylated glycans | 0.69 (0.60 – 0.79) | 2.0E-08 | 0.45 (0.38 – 0.53) | 7.9E-26 | 0.68 (0.62 – 0.76) | 6.9E-14 |
| IgG4_sial | Fraction of sialylated glycans | 1.01 (0.89 – 1.14) | 9.2E-01 | 0.68 (0.59 – 0.78) | 2.2E-08 | 0.66 (0.60 – 0.74) | 3.0E-15 |
| IgG4_A2FB | Bisection of diantennary glycans | 0.79 (0.70 – 0.90) | 3.5E-04 | 0.84 (0.73 – 0.96) | 1.1E-02 | 0.97 (0.88 – 1.08) | 5.9E-01 |
| IgG4_A2FG | Galactosylation per antenna on diantennary glycans | 0.81 (0.71 – 0.92) | 1.5E-03 | 0.48 (0.41 – 0.57) | 2.2E-22 | 0.61 (0.55 – 0.68) | 5.4E-21 |
| IgG4_A2FS | Sialylation per antenna on diantennary glycans | 1.03 (0.90 – 1.17) | 6.8E-01 | 0.70 (0.61 – 0.80) | 1.3E-07 | 0.67 (0.60 – 0.74) | 8.5E-15 |
| IgG4_A2FGS | Sialylation per galactose on diantennary glycans | 1.38 (1.21 – 1.57) | 8.5E-07 | 1.39 (1.22 – 1.60) | 7.2E-07 | 1.00 (0.90 – 1.10) | 9.3E-01 |
a
Derived glycosylation traits were calculated as described in Supp. Table 4.
b
To assess differences between HC and UC patients (HC = 0, UC = 1), HC and CD patients (HC = 0, CD = 1) and between UC and CD patients (UC = 0, CD = 1), logistic regression was performed, using age and sex as co-variables.
c
Displayed are the odds ratios (OR) and their 95 % confidence intervals (CI) for the ITA cohort.
d
p-values were indicated in bold when statistically significant after multiple testing correction (5% FDR). Underlined p-values indicate results that were replicated in the US cohort (Supp. Table 6., 8., and 10.).