Table 1.
Purity and concentration of the extracted genomic DNA
| Method | IT (lysozyme/RNase) | DNA (ng/µL) | DNA (ng/µL) range | A260/280 | A260/280 range | DNA integrity |
|---|---|---|---|---|---|---|
| CLU | 37 °C/37 °C | 309.80 ± 66.79 | 133–448 | 2.083 ± 0.141 | 1.819–2.423 | ++ |
| 60 °C/30 °C | 282.80 ± 85.09 | 50–478 | 2.020 ± 0.161 | 1.701–2.407 | ++ | |
| ZBC | NA | 1002.60 ± 365.15 | 300–2352 | 2.074 ± 0.147 | 1.712–2.413 | + |
| QIA | NA | 161.60 ± 54.35 | 82.5–373 | 2.089 ± 0.096 | 1.810–2.224 | ++ |
Values are represented as the mean ± SE; DNA integrity was assayed by agarose electrophoresis and indicated as good (++) or sufficient (+) based on the band clarity, density and presence of smearing
NA not available, IT incubation temperature