Table 1. Signalment of dogs with squamous cell carcinoma, histopathological subtypes and the results of immunohistochemical examination of periostin.

Case No.	Signalment of dogs			Histopathological Subtypes	Immunohistochemical score and localization of periostinb)						Remark
	Site	Breed	Sex/Agea)		Non-marginal region of cancer			Marginal region of cancer
					BMc)	Mesh-liked)	Diffusee)	BMc)	Mesh-liked)	Diffusee)
1	Nose	Mixed	F/7y	Well-differented	0	0	1+	1+	1+	2+
2	Armpit	Shih Tzu	F/10y	Well-differented	0	0	3+	0	0	3+
3	Eyelid	Labrador Retriever	F/13y	Well-differented	1+	1+	2+	0	0	1+
4	Hind leg	Welsh Corgi	M/10y	Well-differented	0	0	1+	0	0	3+
5	Eyelid	Shetland Sheepdog	F/9y	Well-differented	3+	3+	0	NEf)	NEf)	NEf)	Tru-cutg)
6	Face	Bulldog	M/11y	Well-differented	1+	0	1+	1+	0	1+
7	Chest	Mixed	F/16y	Well-differented	0	0	1+	0	0	3+
8	Nail bed	Bulldog	M/9y	Well-differented	0	0	1+	0	0	1+
9	Abdomen	Great Pyrenees	C/8y	Acantholytic	0	0	1+	0	0	2+
10	Mandible	Jack Russell Terrier	S/11y	Well-differented	0	0	0	0	0	0
11	Nail bed	Labrador Retriever	S/9y	Well-differented	0	0	1+	0	0	2+
12	Penis	Mixed	M/15y	Well-differented	0	0	0	0	0	2+
13	Neck	Shih Tzu	S/9y	Well-differented	0	0	0	0	0	0
14	Armpit	Shiba	C/13y	Well-differented	0	0	0	0	0	1+
15	Nail bed	Mixed	M/unknown	Well-differented	0	0	1+	NEf)	NEf)	NEf)	Small sample
16	Back	Mixed	S/13y	Well-differented	0	0	1+	0	0	3+
17	Neck	Labrador Retriever	M/13y	Well-differented	0	0	1+	0	0	1+
18	Nail bed	Labrador Retriever	M/9y	Well-differented	0	0	0	0	0	0
19	Face	Bernese Mountain Dog	F/11y	Well-differented	0	0	0	0	0	1+
20	Neck	Labrador Retriever	M/11y	Well-differented	0	0	0	0	0	0

a) M, male; F, female; C, castrated male; S, spayed female; y, years old, b) 0<10%, 1+10–50%, 2+50–80%, 3+>80%, c) BM, basement membrane pattern, d) Mesh-like pattern, e) Diffuse pattern, f) NE, Not estimated, g) Biopsy was done by Tru-Cut Needle. It’s difficult to analyse periostin immunolabeling in marginal region of cancer.