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. 2018 Mar 27;10:85. doi: 10.3389/fnagi.2018.00085

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Copyright © 2018 Li, Ma, Wang, Chen, Hu, Wang, Fu, Shi, Zhang and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MSL alleviated mitochondrial dysfunction of copper-induced APPsw cells. (A) Mitochondrial ability was assessed by mitochondrial membrane potential (MMP) and superoxide levels. Fluorescent images of Rh123 and MitoSOX indicated the changes of MMP and superoxide, respectively. Fluorescent images and intensities were analyzed by a Cellomics ArrayScan high-content screening (HCS) Reader and software. Quantified values of Rh123 (B) and MitoSOX (C) were analyzed by Mean_Avginten. Data are expressed as the mean ± SEM, n = 6, **p < 0.01, ***p < 0.001 vs. untreated cells; ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 vs. copper-treated cells.