Table 2.
Detection of cyp51A mutation using Mycogenie® and Aspergenius® assays.
| PCR assay | Number of positive samples (%) for |
|||||
|---|---|---|---|---|---|---|
| A. fumigatus | TR34 | L98H | Y121F | T289A | ||
| AsperGenius® | Target amplificationa | 57 (47.9) | 25/57 (43.9) | 22/57 (38.6) | 30/57 (52.6) | 32/57 (56.1) |
| Detection of mutated alleleb | NA | 0/25 | 0/21 | 0/30 | 0/32 | |
| Mycogenie® | Detection of mutated allele | 64 (53.8) | 0/64 | 0/64 | NA | NA |
NA, not applicable. aSuccessful detection of regions carrying targeted mutations. bMutated cyp51A allele detection achieved by determining melting temperature values from TR34, L98H, Y121F, and T289A region amplicons.