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. 2018 Mar 27;9:210. doi: 10.3389/fphar.2018.00210

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Copyright © 2018 Zhou, Meng, Yang, Hu, Hong, Lv, Chen, Heng, Jiang, Zhu, Cheng, Zhang, Cao, Wang, Shen, Yan and Wu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CEP prevented OVX-induced bone loss in vivo. (A) Representative 3D micro-CT images of trabecular bone of proximal tibias from each group were reconstructed. Scale bar = 1 mm and 500 μm (B–G) The BV/TV, Conn.D, SMI, Tb.N, Tb.Th, and Tb.Sp values of micro-CT data from each sample were obtained and analyzed (H). H&E staining was performed for the histomorphological analysis. Scale bar = 200 μm (I–L) The BV/TV, Tb.N, Tb.Th, and Tb.Sp values of histomorphological sections were measured and quantified. LD and HD represent the dose of 5 and 20 mg/kg CEP, respectively. Values are expressed as mean ± SD, n = 10; ^*P < 0.05, ^**P < 0.01 vs. the control group.