Skip to main content
. 2018 Mar 27;9:210. doi: 10.3389/fphar.2018.00210

Figure 6.

Figure 6

ANI and SC79 partly reversed the inhibitory effects of CEP on RANKL-induced osteoclastogenesis. (A) BMMs were cultured in osteoclastogenic medium containing CEP for 4 days in the presence of DMSO, ANI, and/or SC79. Cells were fixed and TRAP staining was performed. Scale bar = 200 μm. (B) The number and size of TRAP positive osteoclasts were quantified. (C) BMMs were pre-treated with CEP for 2 h and subsequently stimulated with RANKL in the presence of DMSO, ANI, and/or SC79 for 20 min. Cell lysates were collected and analyzed by Western blotting using primary antibodies specific to p-AKT, AKT, p-JNK1/2, and JNK. (D) The gray levels of p-JNK1/2 and p-AKT were analyzed by normalization to total JNK1/2 and AKT. (E) BMMs were cultured with osteoclastogenic medium for 4 days, and subsequently seeded on bone slices with CEP treatment in the presence of DMSO, ANI and/or SC79 for another 48 h. Bone resorption pits were observed under SEM. Scale bar = 200 μm (F) The area of the bone resorption pits was measured. Values are expressed as mean ± SD; *P < 0.05, **P < 0.01 vs. the control group.