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. 2018 Mar 27;9:643. doi: 10.3389/fimmu.2018.00643

Figure 2.

Figure 2

Expression levels of p24 in recombinant Mycobacterium bovis BCG (rBCG) strains and cell lines infected with rBCG strains. (A) Confirmation of p24 expression in rBCG strains using enzyme-linked immunosorbent assay. (B) Confirmation of p24 expression in rBCG strains using a Western blot analysis. Proteins were extracted from wild-type BCG (lane 1) and rBCG strains (lane 2, rBCG-pMV306-p24; lane 3, rBCG-pAL-p24; and lane 4, rBCG-pMyong2-p24). Purified p24 protein was used as a positive control (lane 5). M, molecular weight standard (Elpis Bio, Taejeon, South Korea; DokDo-MARK™). Distinct membranes are separated by white space. In addition, the marker lane is separated by a vertical black line. The expression levels of p24 are plotted. The Western blot image was cropped from a full-length blot to improve clarity. The full-length blot image is presented in the Figure S5 in Supplementary Material. (C) The expression levels of p24 after the infection of the murine macrophage cell line J774A.1 (left panel) and bone-marrow derived dendritic cells (right panel) with wild-type BCG and rBCG strains (rBCG-pMV306-p24, -pAL-p24, and -pMyong2-p24). Data are representative of two independent experiments. Mean ± SD are shown. *P < 0.05; **P < 0.01; ***P < 0.001 (Student’s t-test).