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. 2018 Mar 27;9:643. doi: 10.3389/fimmu.2018.00643

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Copyright © 2018 Kim, Kim, Kook and Kim.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Humoral immune responses induced by p24 recombinant Mycobacterium bovis BCG (rBCG) strains. p24-specific immunoglobulin subtypes (IgG2a, IgG1, and total IgG) were detected by enzyme-linked immunosorbent assay at 450 nm. Optical density (OD) values for the IgG2a and IgG1 subtypes and the ratio of IgG2a/IgG1 were compared. Serum samples from five mice per group were analyzed. Data are representative of two independent experiments. Mean ± SD are shown. *P < 0.05; **P < 0.01; ***P < 0.001 (Student’s t-test).