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. 2018 Mar 27;9:599. doi: 10.3389/fmicb.2018.00599

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Copyright © 2018 dos Santos, Menendez-Gil, Sabharwal, Christensen, Brunhede, Lillebæk and Kallipolitis.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Stress tolerance assay. (A) LO28 wild-type, ΔlhrC1–5 and ΔlisR strains were grown in BHI (Control) and BHI containing 16 μM hemin (Stress). (B) LO28 wild-type, ΔlhrC1–5 and ΔlisR were grown in BHI to OD₆₀₀ = 0.2. Then, the cultures were split in two; one half was stressed with 16 μM hemin (Stress) and the other half kept unstressed (Control). Bacterial growth was monitored until all cultures reached stationary phase. For each assay, the average of three biological replicates is shown.