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. 2018 Mar 27;9:599. doi: 10.3389/fmicb.2018.00599

FIGURE 3.

FIGURE 3

LhrC-mediated down-regulation of the known target genes oppA, tcsA and lapB. (A) Northern blot analysis of oppA mRNA, tcsA mRNA and LhrC1–5. Samples were taken from LO28 wild-type and ΔlhrC1–5 cultures exposed to 8 μM hemin stress for 1 h (+) as well as from non-stressed cultures (–). Northern blots were probed for oppA mRNA, tcsA mRNA, LhrC1–5 and 16S rRNA (loading control). Relative levels of oppA mRNA, tcsA mRNA and LhrC1–5 (normalized to 16S) are shown below each lane. (B) β-galactosidase assay of LO28 wild-type and ΔlhrC1–5 strains carrying a translational reporter gene fusion of lapB to lacZ in the vector pCK-lac (Sievers et al., 2014). β-galactosidase activities of wild-type and mutant cells were measured at the indicated time points under non-stress conditions (Control) and after exposure to 8 μM hemin (Stress). The results are the average of three biological replicates, each carried out in technical duplicates. After 2 h of stress, a significant difference (asterisk) between the mutant and wild-type cells was observed (p < 0.0001).