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. 2018 Mar 27;9:599. doi: 10.3389/fmicb.2018.00599

FIGURE 7.

FIGURE 7

Assessing the effect of LhrC1–5 on the novel target gene lmo0484 upon cefuroxime exposure. (A) β-galactosidase assay of LO28 wild-type and ΔlhrC1–5 strains carrying a translational reporter gene fusion of lmo0484 to lacZ in the vector pCK-lac. β-galactosidase activities of LO28 wild-type and mutant cells were measured at the indicated time points under non-stress conditions (Control) and after exposure to 9 μM cefuroxime (Stress). The results are the average of three biological replicates, each carried out in technical duplicates. After 1 and 2 h of stress, a significant difference between the mutant and wild-type cells was observed (∗∗p < 0.001; ∗∗∗p < 0.0001). (B) Quantification of lmo0484 mRNA in ΔlhrC1–5 relative to LO28 wild-type by RT-qPCR. The ratio of ΔlhrC1–5/LO28 was determined at the 1 h time point for both non-stressed (Control) and cefuroxime-exposed samples (Stress). The result shown is the average of three biological replicates. The asterisk indicates a significant increase of the ratio under stress conditions compared to the control with p < 0.05.