Table 13.

RuBisCO DNA detection in fractions collected during industrial processing of sugar and ethanol production from two types of sugarcane mills.

	Diffuser mill		Tandem roller mill
	Fraction samples	Fraction samples spiked (+)	Fraction samples	Fraction samples spiked (+)
Bagasse	+	+	+	+
Primary juice	+	+	+	+
Filter cake	+	+	+	+
Clarified juice	<LOD	a	+	+
Raw sugar	<LOD	a	<LOD	a
Flegma	<LOD	a	<LOD	a
Vinasse	+	b	+	b
Leaf	+		+

+: positive detection; <LOD: below of limit of detection; spiked (+): samples contaminated with DNA detection of 0.01 ng (nanogram).

^aPositive amplification for samples contaminated with DNA 3 pg of DNA.

^bPositive amplification for samples contaminated with DNA 100 pg of DNA.