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Primers and probes sequences used to identify exogenous (cry1Ab and nptII) and endogenous (ubi) genes present in CTC175-A by qPCR.
| Target | PCR product (pb) | Primer (5′ → 3′) | Primer (5′ → 3′) | Probe |
|---|---|---|---|---|
| cry1ab | 102 | GTGGACAGCCTGGACGAGAT | GAAGCCACTGCGGAACATG | CCCCTCAGAACAAC |
| nptII | 103 | GCTCACCCTGTTGTTTGGTGTT | AGCCTCTCCACCCAAGCG | CTTCTGCAGGTCGACTC |
| ubi1 | 63 | ACCATTACCCTGGAGGTTGAGA | GTCCTGGATCTTCGCCTTCA | CTCTGACACCATCGAC |
