Table 8.
Results of gene amplification of ubi (endogenous gene) and cry1Ab in samples collected during the laboratory sugarcane processing to produce sugar and ethanol from CTC20 and CTC175-A.
| CTC20 + 0.05 ng DNA (cry1ab) |
CTC20 |
CTC175-A |
||||
|---|---|---|---|---|---|---|
| ubi | cry1ab | ubi | cry1ab | ubi | cry1ab | |
| Leaf | + | + | + | − | + | + |
| Bagasse | + | + | + | − | + | + |
| Primary juice | + | + | + | − | + | + |
| Filter cake | + | + | + | − | + | + |
| Clarified juice | + | + | − | − | − | − |
| Syrup | + | + | − | − | − | − |
| Molasses | + | + | + | − | − | − |
| Sugar | + | + | − | − | − | − |
| Flegma | + | + | − | − | − | − |
| Vinasse | + | + | <LOD | − | <LOD | <LOD |
| Evaporation water | + | + | − | − | − | − |
| Condensation water | + | + | − | − | − | − |
Samples obtained from cultivar CTC20 were intentionally spiked with 0.5 and 0.05 ng of DNA from CTC175-A event. +: presence; −: absence; <LOD: the amplification curve shows cq later than the same sample at a concentration of 0.05 ng of total DNA per reaction.