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. 2018 Mar 27;8:95. doi: 10.3389/fcimb.2018.00095

Figure 2.

Figure 2

Ag uptake, migration, and Ag presentation capability of DCs by GrpE treatment. (A–C) DCs were treated with GrpE or LPS for 24 h. (A) Cells were incubated with dextran-FITC at 37 and 4°C for 30 min, and assessed by flow cytometry. The percentages of dextran+CD11c+ cells are indicated. One representative plot from three independent experiments is shown. (B) CCR7 expression in DCs (on gated CD11c+) was analyzed by flow cytometry. (C) DCs were treated with GrpE and the relative intensity of migrated cells was identified through CCL19. Bar graphs display mean ± SD (n = 3 samples) and statistical significance (*p < 0.05, **p < 0.01, or ***p < 0.001) is shown for treatments compared to non-treated DCs (Control). (D,E) DCs were treated with LPS or GrpE, and then pulsed with OVA protein or Eα44–76 peptide. After 24 h, cells were stained with anti-CD11c, anti-25-D1.16, or anti-Y-Ae mAbs. Histogram and bar graphs for expression of OVA257–264/H-2Kb (D) and Eα52–68/I-Ab complexes (E) are displayed. OVA257–264 or Eα52–68 peptide (each peptide; 2 μg/mL concentration) was used as a positive control for antigen presentation. Histogram data are representative result of three experiments. Bar graph data is expressed as the mean ± SD (n = 3 samples); ***p < 0.001; Con denotes untreated DCs.