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Competitive EMSAs for calculation of binding affinities of the CcpA protein with the creP and creORF sites. (A) Competitive effect of creP on CcpA-creORF binding. (B) Competitive effect of creORF on CcpA-creP binding. The initial concentration of Cy5-Df1 and Cy5-Df2 DNA was 0.04 pM, and 1.0 μM CcpA was used.
