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Effects of PccD on erythromycin production in S. erythraea NRRL2338. Supernatants (cultivated in industrial medium plus 30 mM valine) were collected after being cultivated for about 6 days. Erythromycin was measured by HPLC as described in Materials and Methods. Three independent replicates were used to calculate the standard deviations. **, P < 0.01; ****, P < 0.0001.
