Fig. 1. MiR-144-3p is a TE-targeting miRNA highly expressed in mouse and human ASMC.
(A) A representative flow chart for negative selection of mouse aortic cells for CD31 by flow cytometry to remove endothelial cells. (B, C) The purified ASMC (MASMC) was stained for alpha smooth muscle actin (α-SMA; B) and calponin (C). (D, E) Expression of candidate TE-targeting miRNAs (miR-29, miR-101, miR-181-5p and miR-144-3p) in human ASMC (HASMC; D) and in MASMC (E) by RT-qPCR. *p < 0.05. NS: non-significant. N = 5.