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. 2018 Feb 21;41(3):234–243. doi: 10.14348/molcells.2018.2340

Fig. 3. Activation of cAMP/PKA/β-catenin and PI3K/AKT/GSK3β/β-catenin signaling pathway in liraglutide-stimulated osteoblastic MC3T3-E1 cells.

Fig. 3

Cells were exposed to 100 nM liraglutide and 20 μM H89 or 20 μM LY294002, respectively. Cell lysates were subjected to Western blotting and ELISA to assess AKT (A, B), GSK3β (A, C), β-catenin (D, E), cAMP (F) activation. β-catenin level in the cytoplasm and nucleus (G–I) and TCF7L2 mRNA expression (J) were detected to evaluate the canonical Wnt/β-catenin signaling pathway activation.