Morphological phenotype of lec2 mutants.
(A) lec2-5,
(C) lec2-3, and (E,
G) lec2-4 mutant embryos.
(B, D, F, H) Wild-type embryo. (A and
B) Whole-mount photographs of maturing embryos.
(C and D) Embryonic suspensors as viewed
by using differential interference contrast (DIC) microscopy of cleared
seeds. Arrows point to abnormal suspensor cells in lec2
mutants. (E and F) Cotyledons of
seedlings grown for 4–5 days. A lec2 mutant seedling
germinated before desiccation possessed trichomes on the adaxial
surface of cotyledons. (G and H) Shoot
apices of curled cotyledon-stage embryos seen with DIC optics. The
shoot apical meristem of lec2 mutants is domed and
possesses leaf primordia in contrast to the unactivated meristem of
wild types. ep, Embryo proper; p, leaf primordium; SAM, shoot apical
meristem; s, suspensor. [Bars = 100 μm (A), 20
μm (C, G), 300 μm (E).]