Figure 4.

LEC2 RNA accumulates primarily during seed development. LEC2 RNA was detected at the indicated stages by amplifying reverse transcription products. Silique stages 1 through 4, respectively, are from siliques containing zygote to early globular-stage embryos, globular-stage to heart-stage embryos, torpedo-stage to curled cotyledon-stage embryos, and mature green embryos. Control experiments showed that a ribosomal protein RNA was amplified with similar efficiency from each reverse transcription reaction.