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. 2001 Sep 25;98(20):11806–11811. doi: 10.1073/pnas.201413498

Figure 5.

Figure 5

LEC2 induces somatic embryo development. Seeds from lec2-1 and lec2-5 plants transformed with the 35S∷LEC2 gene were germinated, and their subsequent development was monitored. (A) Formation of somatic embryo-like clusters (arrows) on the cotyledons of an embryo-like 35S∷LEC2 seedling. (B) Somatic embryo-like structures emerging from the cotyledon (arrowhead) of a 35S∷LEC2 seedling. (C) Scanning electron microscopy (SEM) photograph of a wild-type linear cotyledon-stage zygotic embryo. (D) A mass of somatic embryo-like structures covering a 35S∷LEC2 seedling as observed by SEM. (E) SEM photograph of somatic embryo-like structures on a 35S∷LEC2 seedling. (F) Seedling resulting from the germination of a 35S∷LEC2 somatic embryo formed on a wild-type transgenic seedling. (G) A 35S∷LEC2 seedling with a wild-type phenotype. Arrowhead shows that the distal region of the cotyledon is not defective. (H) A short, bushy plant grown on soil that developed from a 35S∷LEC2 seedling such as that shown in G. (I) Somatic embryos (arrows) emerging from leaf-like organ dissected from a plantlet mass. (J) A mass of plantlets formed from a single 35S∷LEC2 seedling such as that shown in G. a, Embryonic axis; al, embryonic axis-like; c, cotyledon; cl, cotyledon-like. [Bars = 1 mm (A, B, D, F, G), 100 μm (C, E), 500 μm (I), 1 cm (J), and 5 cm (H).]