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. 2001 Sep 25;98(20):11806–11811. doi: 10.1073/pnas.201413498

Figure 6.

Figure 6

LEC2-induced somatic embryos express embryo-specific genes. (A) Whole-mount photograph of a 35S∷LEC2 seedling with somatic embryo-like structures on its cotyledons. Sections of the same seedling were hybridized with antisense probes for LEC2 RNA (B), cruciferin A storage protein RNA (C), and oleosin lipid body protein RNA (D). After autoradiography, sections were photographed with use of dark-field optics. A sense-strand probe did not hybridize appreciably with the sections (data not shown). Regions of the seedlings in B–D that did not hybridize lacked cellular contents and were likely dead. c, Cotyledon; se, somatic embryo. (Bars = 0.5 mm.)