FIG. 3.

Arsenic inhibits wound healing in an in vitro model, and estrogen treatment counteracts arsenic's effects. hDFn were grown in 12-well plates and incubated until the cells reached 100% confluence. The cells were scratched by using a P200 pipette tip, and the wells were imaged every 4 hours for 24 hours to determine the degree to which the cells filled in the scratch space. The % closure was determined at each time point, and the AUC was determined for summed % closure across all time points as a measure of wound healing. A significant two-way ANOVA interaction effect was found (p < 0.001). Statistical significance is shown by using the “compact letter display” notation, commonly used in statistical analysis with multiple comparisons. Treatments that do not share a common letter are statistically significant from each other (p < 0.05). Treatments distinguished by different letters are significantly different by a Tukey's post hoc analysis (p < 0.05). ANOVA, analysis of variance.