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. 2018 Mar 27;8:92. doi: 10.3389/fcimb.2018.00092

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Copyright © 2018 da Silva, Menezes, Kitano, Oliveira, Abreu, Souza, Heinemann, Isaac, Fraga, Serrano and Barbosa.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Proteolytic activity correlates with virulence. Supernatants of (1) L. biflexa serovar Patoc strain Patoc I, (2) L. interrogans serovar Kennewicki strain Fromm (LPF), (3) L. biflexa serovar Andamana strain CH11, (4) L. interrogans serovar Copenhageni strain 10A, (5) L. interrogans serovar Pomona strain Pomona, (6) L. kirshneri serovar Cynopteri strain 3522 C, (7) L. noguchi serovar Panama CZ 214 (0.5 μg of total secreted proteins) were incubated with plasma fibronectin (5 μg), fibrinogen (15 μg), and decorin (2 μg) for 18 h h at 37°C. Cleavage products were analyzed by SDS- polyacrylamide gel under reducing conditions. Gels were silver stained. FN, plasma fibronectin; FBG, fibrinogen; DCN, decorin. Lane 8: controls (purified substrates).