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. Author manuscript; available in PMC: 2018 Apr 3.
Published in final edited form as: J Med Chem. 2017 Nov 14;60(22):9290–9298. doi: 10.1021/acs.jmedchem.7b01280

Figure 4.

Figure 4

(A) Structure of lead compound 1. (B) In vitro kinase assay was performed using 50 ng of EphA2 and 50 μg of poly(Glu-Tyr) as a substrate containing 2 μM 1. Quantification of substrate phosphorylation was shown. (C) Autophosphorylation of EphA2 was detected by incubating 50 ng of EphA2 with 2 μM 1 in the kinase assay buffer. Immunoblot analysis with pTyr antibody was performed. (D) C13 cells were treated with the indicated concentrations of 1 for 24 h. Immunoblot analysis of EphA2-Ser897 was performed. Total EphA2 was used as a control. The extent of phosphorylation was quantified using ImageJ software. Densitometric analysis was performed by SPSS.