Skip to main content
. Author manuscript; available in PMC: 2019 Apr 1.
Published in final edited form as: Trends Genet. 2018 Jan 9;34(4):270–278. doi: 10.1016/j.tig.2017.12.010

Figure 1. Junctional tag.

Figure 1

Schematic representation of eccDNA and junctional sequence genesis from linear DNA. The two ends of linear DNA get ligated and ligation event creates a new junctional sequence which is not present in parent linear DNA; The sequencing reads partially mapping to the left and right side of ligation point are the junctional sequence. Because the junctional sequence is not present in linear DNA it acts as a discriminatory feature within computer algorithms to validate that the DNA fragment was within a circular DNA molecule [27, 28, 49]. The paired end reads pairs where one read completely maps inside the body of circle and other second reads maps on the junctional sequence are used for the final validation of circular nature of the starting DNA fragment.