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. 2018 Apr 2;215(4):1101–1113. doi: 10.1084/jem.20170084

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© 2018 Dobenecker et al.

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Figure 2. — PRC2 is essential for TCR-mediated T cell proliferation and transcription of IL2 and IL2RA in vitro. (A) The histograms show the pattern of CFSE dilution between WT or mutant splenic CD4⁺ T cells triggered by indicated stimuli for 72 h. The histograms marked in gray and blue show amount of CFSE in nonstimulated cells or stimulated cells, respectively. (B) The amounts of viable T cells at different time points after stimulation with anti-CD3/anti-CD28 or PMA/ionomycin (Iono) are shown. (C and D) The expression levels of IL-2 (C) and IL-2RA (D) in TCR-triggered WT and PRC2-deficient splenic T cells were measured by qPCR at different time points after T cell stimulation. All data are representative of three independent experiments (n = 3–6) and significance was determined by the unpaired Student’s t test: *, P ≤ 0.05; **, P ≤ 0.01; and ***, P ≤ 0.001. Error bars show mean ± SEM.