Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Apr 2;215(4):1115–1133. doi: 10.1084/jem.20171608

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 Baranska et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 2. — Replenishment kinetics of blood monocytes of CD64^dtr mice after DT treatment. (A) Gating strategy used to identify CD11b⁺Lin^– cell subsets within the blood. Red blood cells were lysed before analysis, and the absolute number of a given cell type present in a given blood volume was determined by staining samples in Trucount Absolute Counting Tubes. Beads were gated out on the basis of their FSC-A–SSC-A profile, and after excluding CD5⁺ T cells, CD19⁺ B cells, CD161⁺ NK cells, as well as SSC-A^int eosinophils, and Ly-6G⁺ neutrophils, the majority of the remaining CD11b⁺Lin^– cells were CD45⁺CD115⁺; when analyzed on CD115-CD43 dot plots, they comprise CD115⁺CD43^– and CD115⁺CD43⁺ subsets. As expected, CD115⁺CD43^– cells corresponded to Ly-6C^high blood monocytes, whereas most CD115⁺CD43⁺ corresponded to Ly6-C^low blood monocytes. (B) Absolute number (mean values ± SD) of the specified cells in the blood of CD64^dtr mice before and 1, 2, 3, 4, 5, 10, and 20 d after DT treatment. (C) CD115⁺CD43^– monocytes were analyzed at the specified time points by using Ly-6C–MHCII dot plots to define the percentages of Ly-6C^highMHCII^– and Ly-6C^highMHCII⁺ monocytes. (D) CD115⁺CD43⁺ cells were analyzed at the specified time points by using Ly-6C–MHCII dot plots to define the percentages of Ly-6C⁺MHCII^– and Ly-6C^lowMHCII^– monocytes. (E) Ly-6G^–SSC-A^low cells were analyzed at the specified time points by using CD115-CD45 dot plots to define the percentages of CD115^int and CD115⁺ blood monocytes. Data are representative of at least three experiments involving three to six animals per group.