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. 2018 Apr 2;217(4):1503–1519. doi: 10.1083/jcb.201702048

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© 2018 Laban et al.

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Figure 5. — Increased polarization of VASP^−/− leukocytes in vivo. qPCR analysis of infiltrated leukocytes, isolated from the ischemic thigh and calf muscles from WT (+/+) and VASP^−/− (−/−) mice 3 d after femoral artery excision. (A) Expression of the proinflammatory genes iNOS, TNF-α, and IL-1β. (B) Expression of the anti-inflammatory genes IL-10, arginase 1, and Ym1. (C) Expression of the hypoxia-related genes HIF1α, HIF2α, and VEGF. n = 5 animals per group, error bars, SEM; *, P < 0.05; **, P < 0.01; ***, P < 0.001 (Student’s t test).