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. 2018 Apr 2;217(4):1453–1465. doi: 10.1083/jcb.201707055

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© 2018 Sun et al.

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Figure 6. — Disruption of GALT development in Itgb7^L720P/L720P mice. (A) Schematic of the Cas9/sgRNA-targeting sites in Itgb7. The sgRNA-targeting sequences are underlined in blue, and the protospacer-adjacent motif (PAM) sequence is labeled in blue in the WT sequence. The MaeI restriction site removed from the WT and ApaI site introduced in the mutant are labeled as are the Leu codon changed to Pro. (B) Sequencing analysis of WT and β7(L720P) knock-in mice. DNA sequencing confirmed a leucine to proline substitution at position 720 of the mouse β7 integrin gene (position 721 in human β7 integrin gene). The mutation is labeled in red. The silent mutations are labeled in green and with green asterisks. (C) The absolute number of CD3⁺ T cells and B220⁺ B cells isolated from Itgb7^WT/WT mice and Itgb7^WT/L720P, or Itgb7^L720P/L720P littermates are shown. Error bars show means ± SD. n = 8. NS, P > 0.05; ***, P < 0.001.