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. 2018 Apr 2;217(4):1319–1334. doi: 10.1083/jcb.201708179

Figure 2.

Figure 2.

KIF27 and KIF7 can inhibit microtubule growth in vitro. (A) Schematic of the microtubule dynamics assay. Microtubule seeds generated from biotin-tubulin and HiLyte647-tubulin and polymerized in GMPCPP were attached to the slide, and then microtubule polymerization was performed in the presence of 10.7 µM tubulin and 1 mM GTP. (B and E) Representative kymographs of microtubules polymerizing in the absence (0 nM) or presence of indicated concentrations of KIF27 (B) or KIF7 (E). HiLyte647 microtubule seeds are in red. X-rhodamine dynamic microtubules are in green. Time is on the x axis (bar, 5 min), and distance is on the y axis (bar, 2 µm). (C and F) Plots of microtubule growth rate measured in the absence or presence of the indicated concentrations of KIF27 (C) or KIF7 (F). (D and G) Plots of microtubule growth length before catastrophe measured in the absence or presence of indicated concentrations of KIF27 (D) or KIF7 (G). Scatterplots display means ± SEM (n = 20–43 for each condition) from two or three independent experiments.