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. 2018 Apr 2;217(4):1319–1334. doi: 10.1083/jcb.201708179

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© 2018 Yue et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

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Figure 5. — Multimotor properties of kinesin-4 motors in microtubule gliding assays. (A) Schematic of microtubule gliding assay. Truncated dimeric kinesin-4 motors were tagged at their C termini with the AviTag and biotinylated by coexpression with the biotin ligase BirA. The biotinylated motors were bound to the surface of a NeutrAvidin-coated flow cell, and fluorescent microtubules were added. (B–E) Representative kymographs show microtubule gliding driven by the indicated motors (top). Time is on the x axis, and distance is on the y axis. The velocity of each microtubule gliding event was determined via kymograph analysis and plotted as a histogram for each population (bottom). The curves were fit with a normal distribution. Gliding speeds are described as means ± SEM. N, number of motility events analyzed across three independent experiments.