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. 2018 Apr 2;217(4):1319–1334. doi: 10.1083/jcb.201708179

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© 2018 Yue et al.

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Figure 6. — Multimotor properties of kinesin-4 motors in a cellular transport assay. (A) Schematic of the inducible peroxisome redistribution assay. Truncated dimeric kinesin motors tagged with the fluorescent protein mCit and FRB were coexpressed with the peroxisome-targeted PEX-mRFP-FKBP fusion protein in COS-7 cells. Addition of rapamycin causes heterodimerization of FRB and FKBP and thereby recruits kinesin motors to the peroxisome surface. Recruitment of active motors causes redistribution of peroxisomes to the cell periphery. (B–E) Representative images of peroxisome distribution in COS-7 cells expressing the indicated kinesin motors before (0 min; top) and after (25 min; middle) addition of rapamycin (rap). Yellow lines indicate the periphery of each cell; white dotted lines indicate the nucleus. Bar, 10 µm. Representative graphs (bottom) show the peroxisome distribution over time upon recruitment of the indicated kinesin-4 motors in a representative cell. Each blue dotted line represents the peroxisome distribution at that time point. The value was normalized by the peroxisome distance from center in the first frame. The black arrow indicates the time point of rapamycin addition.